Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Expression of a mutant IRS inhibits metabolic and mitogenic signalling of insulin in human adipocytes.

Molecular and cellular endocrinology - 30 Jun 2004

Stenkula Karin G, Said Lilian, Karlsson Margareta, Thorn Hans, Kjølhede Preben, Gustavsson Johanna, Söderström Mats, Strålfors Peter, Nystrom Fredrik H

Abstract excerpt

Adipose tissue is a primary target of insulin, but knowledge about insulin signalling in human adipocytes is limited. We developed an electroporation technique for transfection of primary human adipocytes with a transfection efficiency of 15% +/- 5 (mean +/- S.D.). Human adipocytes were co-transfected with a mutant of IRS-3 (all four potential PI3-kinase binding motifs mutated: IRS-3F4) and HA-tagged protein...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to 3-Phosphoinositide-Dependent Protein Kinases
Share to Adipocytes

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Electroporation

Share to ets-Domain Protein Elk-1

Share to Glucose Transporter Type 4

Share to Insulin Receptor Substrate Proteins

Share to Monosaccharide Transport Proteins

Share to Muscle Proteins

Source

Identifiers

PMID: 15223127
DOI: 10.1016/j.mce.2004.04.011

Genes

SDS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Expression of a mutant IRS inhibits metabolic and mitogenic signalling of insulin in human adipocytes.PMID 15223127Read ↗

Action of insulin receptor substrate-3 (IRS-3) and IRS-4 to stimulate translocation of GLUT4 in rat adipose cells.Semantic proximity Read ↗

Roles of 1-phosphatidylinositol 3-kinase and ras in regulating translocation of GLUT4 in transfected rat adipose cells.Semantic proximity Read ↗

The G972R variant of the insulin receptor substrate-1 gene impairs insulin signaling and cell differentiation in 3T3L1 adipocytes; treatment with a PPARgamma agonist restores normal cell signaling and differentiation.Semantic proximity Read ↗

Development of a new model system to dissect isoform specific Akt signalling in adipocytes.Semantic proximity Read ↗

Requirement of atypical protein kinase clambda for insulin stimulation of glucose uptake but not for Akt activation in 3T3-L1 adipocytes.Semantic proximity Read ↗

Positive and negative roles of p85 alpha and p85 beta regulatory subunits of phosphoinositide 3-kinase in insulin signaling.Semantic proximity Read ↗

High Efficiency Lipid-Based siRNA Transfection of Adipocytes in SuspensionSemantic proximity Read ↗

Requirement for PIKfyve enzymatic activity in acute and long-term insulin cellular effects.Semantic proximity Read ↗

Protein kinase B phosphorylation of PIKfyve regulates the trafficking of GLUT4 vesicles.Semantic proximity Read ↗

Mechanisms regulating phosphoinositide 3-kinase signalling in insulin-sensitive tissues.Semantic proximity Read ↗

Fatty acid-binding protein 3 stimulates glucose uptake by facilitating AS160 phosphorylation in mouse muscle cells.Semantic proximity Read ↗

Caveolin-2 regulation of STAT3 transcriptional activation in response to insulin.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.