Article
Integrative analysis of the mitochondrial proteome in yeast.
PLoS biology - 1 Jun 2004
Prokisch Holger, Scharfe Curt, Camp David G, Xiao Wenzhong, David Lior, Andreoli Christophe, Monroe Matthew E, Moore Ronald J, Gritsenko Marina A, Kozany Christian, Hixson Kim K, Mottaz Heather M, Zischka Hans, Ueffing Marius, Herman Zelek S, Davis Ronald W, Meitinger Thomas, Oefner Peter J, Smith Richard D, Steinmetz Lars M
Abstract excerpt
In this study yeast mitochondria were used as a model system to apply, evaluate, and integrate different genomic approaches to define the proteins of an organelle. Liquid chromatography mass spectrometry applied to purified mitochondria identified 546 proteins. By expression analysis and comparison to other proteome studies, we demonstrate that the proteomic approach identifies primarily highly abundant proteins....
Topics
- Chemical Fractionation
- Chromatography, Liquid
- Gene Expression Profiling
- Genome
- Mass Spectrometry
- Mitochondria
- Mitochondrial Proteins
- Phenotype
- Proteomics