Article
High-resolution genome-wide scan of genes, gene-networks and cellular systems impacting the yeast ionome.
BMC genomics - 14 Nov 2012
Yu Danni, Danku John M C, Baxter Ivan, Kim Sungjin, Vatamaniuk Olena K, Vitek Olga, Ouzzani Mourad, Salt David E
Abstract excerpt
BACKGROUND: To balance the demand for uptake of essential elements with their potential toxicity living cells have complex regulatory mechanisms. Here, we describe a genome-wide screen to identify genes that impact the elemental composition ('ionome') of yeast Saccharomyces cerevisiae. Using inductively coupled plasma - mass spectrometry (ICP-MS) we quantify Ca, Cd, Co, Cu, Fe, K, Mg, Mn, Mo, Na, Ni, P, S and Zn...
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