Article
Subcellular localization of the yeast proteome.
Genes & development - 15 Mar 2002
Kumar Anuj, Agarwal Seema, Heyman John A, Matson Sandra, Heidtman Matthew, Piccirillo Stacy, Umansky Lara, Drawid Amar, Jansen Ronald, Liu Yang, Cheung Kei-Hoi, Miller Perry, Gerstein Mark, Roeder G Shirleen, Snyder Michael
Abstract excerpt
Protein localization data are a valuable information resource helpful in elucidating eukaryotic protein function. Here, we report the first proteome-scale analysis of protein localization within any eukaryote. Using directed topoisomerase I-mediated cloning strategies and genome-wide transposon mutagenesis, we have epitope-tagged 60% of the Saccharomyces cerevisiae proteome. By high-throughput immunolocalization...
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