Article
A point mutation of c-Ki-ras gene was found in human esophageal carcinoma cell lines but not in primary esophageal carcinomas.
Biochemical and biophysical research communications - 31 Aug 1992
Shiga C, Shiga K, Sasano H, Mori S
Abstract excerpt
Activation of the ras oncogene in primary human esophageal carcinomas and cell lines established from such carcinomas was analyzed using the polymerase chain reaction (PCR)-direct sequencing method. This analysis revealed a GC----AT transition at the second base in the 12th codon of the c-Ki-ras gene in TE1 and TE2 esophageal carcinoma cell lines. In contrast, no point mutation was detected in the 12th, 13th, and...
Topics
- Adenocarcinoma
- Base Sequence
- Carcinoma, Squamous Cell
- Codon
- DNA, Neoplasm
- Esophageal Neoplasms
- Genes, ras
- Humans
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction