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Article

Improved generation of C57BL/6J mouse embryonic stem cells in a defined serum-free media.

Genesis (New York, N.Y. : 2000) - 1 Jun 2004

Cheng Jun, Dutra Amalia, Takesono Aya, Garrett-Beal Lisa, Schwartzberg Pamela L

Abstract excerpt

C57BL/6 is a well-characterized mouse strain that is used extensively for immunological and neurological research. The establishment of C57BL/6 ES cell lines has facilitated the study of gene-altered mice in a pure genetic background-however, relatively few such lines exist. Using a defined media supplement, knockout serum replacement (KSR) with knockout DMEM (KSR-KDMEM), we find that we can readily establish ES...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 15170695
DOI: 10.1002/gene.20031

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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