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A simple and economic protocol for efficient in vitro fertilization using cryopreserved mouse sperm

28 Oct 2021

Abstract excerpt

The advent of genome editing tools like CRISPR/Cas has substantially increased the number of genetically engineered mouse models in recent years. In support of refinement and reduction, sperm cryopreservation is advantageous compared to embryo freezing for archiving and distribution of such mouse models. The in vitro fertilization using cryopreserved sperm from the most widely used C57BL/6 strain has become...

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PMID: 34710162
DOI: 10.1371/journal.pone.0259202

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