Article
In situ lacZ retrovirus-marked lymphocytes define a B cell microenvironment in the lymph node medulla.
The New biologist - 1 May 1992
Krall W J, Braun J
Abstract excerpt
A recombinant nonreplicating retroviral vector bearing the Escherichia coli lacZ indicator gene was used to mark a population of B cells in situ in murine lymphoid tissue. The retrovirus was surgically injected into popliteal lymph nodes during the primary immune response to DNP-CGG when B cell proliferation in the germinal centers was maximal. LacZ+ cells were initially detected in the perivascular medullary...
Topics
- Animals
- B-Lymphocytes
- Base Sequence
- Cell Differentiation
- Cell Movement
- DNA
- Female
- Immunoglobulin kappa-Chains
- Immunologic Memory
- Lac Operon
- Lymph Nodes
- Mice