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Article

ZyFISH: a simple, rapid and reliable zygosity assay for transgenic mice.

PloS one - 1 Jan 2012

McHugh Donal, O'Connor Tracy, Bremer Juliane, Aguzzi Adriano

Abstract excerpt

Microinjection of DNA constructs into fertilized mouse oocytes typically results in random transgene integration at a single genomic locus. The resulting transgenic founders can be used to establish hemizygous transgenic mouse lines. However, practical and experimental reasons often require that such lines be bred to homozygosity. Transgene zygosity can be determined by progeny testing assays which are expensive...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 22666404
DOI: 10.1371/journal.pone.0037881
PMCID: PMC3362593

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