Article
Allele-specific and asymmetric polymerase chain reaction amplification in combination: a one step polymerase chain reaction protocol for rapid diagnosis of familial defective apolipoprotein B-100.
Analytical biochemistry - 1 Jul 1992
Schuster H, Rauh G, Müller S, Keller C, Wolfram G, Zöllner N
Abstract excerpt
We have combined the asymmetric polymerase chain reaction (PCR) with allele-specific PCR to detect a single point mutation. A set of two priming oligonucleotides and a third allele-specific primer were used to identify heterozygotes for a G to A mutation at nucleotide 10,708 in the apolipoprotein B (apo B) gene. The system requires neither restriction enzyme digestion nor allele-specific oligonucleotides as...
Topics
- Alleles
- Apolipoprotein B-100
- Apolipoproteins B
- Base Sequence
- DNA
- DNA Mutational Analysis
- Female
- Humans
- Hyperlipoproteinemias
- Male
- Molecular Sequence Data