Allele-specific competitive blocker PCR: a one-step method with applicability to pool screening.

doi:10.1002/humu.1380060209

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Article

Allele-specific competitive blocker PCR: a one-step method with applicability to pool screening.

Human mutation - 1 Jan 1995

Orou A, Fechner B, Utermann G, Menzel H J

Abstract excerpt

We have developed a novel one-step pool screening PCR procedure which is based on the principles of amplification refractory mutation system (ARMS) and competitive oligonuleotide priming (COP) PCR. In addition to the usual primers, this approach uses two allele-specific competitive oligonucleotid...

Topics

Alleles
Base Sequence
Cystic Fibrosis Transmembrane Conductance Regulator
Genetic Testing
Humans
Lipoprotein Lipase
Molecular Sequence Data
Mutation
Polymerase Chain Reaction

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