Rapid typing of apolipoprotein B DNA polymorphisms by DNA amplification. Association between Ag epitopes of human apolipoprotein B-100, a signal peptide insertion/deletion polymorphism, and a 3'flanking DNA variable number of tandem repeats polymorphism of the apolipoprotein B gene.

Abstract excerpt

We present here rapid and efficient methods for the analysis of multiple variable apolipoprotein (apo) B loci using polymerase chain reaction based techniques. For illustrative purposes, we have applied these methods to establish an association between these polymorphisms and the apo B Ag immunological epitopes. The 5 DNA polymorphisms include 3 restriction endonuclease sites (for XbaI, EcoRI and MspI), a...

Topics

• Alleles
• Apolipoprotein B-100
• Apolipoproteins B
• Base Sequence
• DNA
• Epitopes
• Humans
• Molecular Sequence Data
• Polymerase Chain Reaction
• Polymorphism, Genetic