Article
Specifically associated PCR products probed by coincident detection of two-color cross-correlated fluorescence intensities in human gene polymorphisms of methylene tetrahydrofolate reductase at site C677T: a novel measurement approach without follow-up mathematical analysis.
Experimental and molecular pathology - 1 Jun 2004
Földes-Papp Zeno, Costa Jean M, Demel Ulrike, Tilz Gernot P, Kinjo Masataka, Saito Kenta, Kii Hiroaki, Takagi Takuya, Tamura Mamoru, Thyberg Per, Birch-Hirschfeld Eckhard
Abstract excerpt
Whole blood samples of known methylene tetrahydrofolate reductase (MTHFR) genotypes from 24 individuals were examined at site C677T. Their amplified DNA products were assessed by two-color fluorescence cross-correlation measurements and agarose gel electrophoresis/capillary gel electrophoresis. DNA subpopulations were identified which were not associated with the proper genotype by primer combinations and cycling...
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