Article
C677T mutation of methylenetetrahydrofolate reductase gene determined in blood or plasma by multiple-injection capillary electrophoresis and laser-induced fluorescence detection.
Clinical chemistry - 1 Feb 1997
Ulvik A, Refsum H, Kluijtmans L A, Ueland P M
Abstract excerpt
We constructed an assay to detect the common C677T mutation in the methylenetetrahydrofolate reductase gene. The mutation creates a Hinfl recognition site detected by restriction cleavage of a 198-bp fragment amplified in the polymerase chain reaction (PCR). Digested samples were subjected to capillary electrophoresis with laser-induced fluorescence detection (CE-LIF), with hydroxypropylmethylcellulose as the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.