Article
Directed evolution of protein enzymes using nonhomologous random recombination.
Proceedings of the National Academy of Sciences of the United States of America - 4 May 2004
Bittker Joshua A, Le Brian V, Liu Jane M, Liu David R
Abstract excerpt
We recently reported the development of nonhomologous random recombination (NRR) as a method for nucleic acid diversification and applied NRR to the evolution of DNA aptamers. Here, we describe a modified method, protein NRR, that enables proteins to access diversity previously difficult or impossible to generate. We investigated the structural plasticity of protein folds and the ability of helical motifs to...
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