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Proofreading genotyping assays and electrochemical detection of SNPs.

Human mutation - 1 May 2004

King Garry C, Di Giusto Daniel A, Wlassoff Wjatschesslaw A, Giesebrecht Susanne, Flening Eleanor, Tyrelle Gregory D

Abstract excerpt

The use of proofreading DNA polymerases in genotyping assays offers the prospect of improved performance. To this end, we have recently used compatible DNA polymerases, protected primers, and substrates to implement proofreading single base extension (P-SBE) and proofreading allele-specific extension (PRASE) assays. Key aspects of the P-SBE and related proofreading assay formats are described here. For...

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PMID: 15108272
DOI: 10.1002/humu.20034

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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