Article
A single base extension technique for the analysis of known mutations utilizing capillary gel electrophoreisis with electrochemical detection.
Analytical chemistry - 15 Jul 2002
Brazill Sara A, Kuhr Werner G
Abstract excerpt
A novel single nucleotide polymorphism (SNP) detection system is described in which the accuracy of DNA polymerase and advantages of electrochemical detection are demonstrated. A model SNP system is presented to illustrate the potential advantages in coupling the single base extension (SBE) technique to capillary gel electrophoresis (CGE) with electrochemical detection. An electrochemically labeled primer, with a...
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