Article
HLA-B27 typing: evaluation of an allele-specific PCR melting assay and two flow cytometric antigen assays.
Cytometry. Part B, Clinical cytometry - 1 Jan 2005
Seipp Michael T, Erali Maria, Wies Rae Lynn, Wittwer Carl
Abstract excerpt
BACKGROUND: Human leukocyte antigen B27 (HLA-B27) is a major histocompatibility complex class 1 molecule that is strongly associated with the disease ankylosing spondylitis. Testing for HLA-B27 is of diagnostic value because 90% of patients with ankylosing spondylitis have the B27 antigen. Two commonly used HLA-B27 flow cytometric assays are commercially available. METHODS: An allele-specific polymerase chain...
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