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Article

Minor groove binder-conjugated DNA probes for quantitative DNA detection by hybridization-triggered fluorescence.

BioTechniques - 1 Apr 2002

Afonina I A, Reed M W, Lusby E, Shishkina I G, Belousov Y S

Abstract excerpt

Here we describe the properties of a novel class of oligonucleotide probes capable of sensitive hybridization-triggered fluorescence. These fluorogenic probes, known commercially as MGB Eclipse probes, are characterized by having a conjugated minor groove binder (MGB) ligand at the 5'-end and a fluorophore at the 3'-end. Additionally, they have an efficient quencher moiety at the 5'-end that is useful with a wide...

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PMID: 11962616
DOI: 10.2144/02324pf01

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