Article
Cloning of the Rhizopus niveus pyr4 gene and its use for the transformation of Rhizopus delemar.
Current genetics - 1 Apr 1995
Horiuchi H, Takaya N, Yanai K, Nakamura M, Ohta A, Takagi M
Abstract excerpt
We have cloned a pyr4 gene encoding orotidine-5'-monophosphate decarboxylase of the filamentous fungus Rhizopus niveus. The pyr4 gene of R. nivens has an open reading frame composed of 265 amino-acid residues and has two putative introns. We have also isolated a pyr4 mutant of Rhizopus delemar from 5-fluoroorotic acid-resistant mutants and transformed it with the pyr4 gene of R. niveus as a selectable marker....
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