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Article

Systematic insertional mutagenesis of a streptomycete genome: a link between osmoadaptation and antibiotic production.

Genome research - 1 May 2004

Bishop Amy, Fielding Sue, Dyson Paul, Herron Paul

Abstract excerpt

The model organism Streptomyces coelicolor represents a genus that produces a vast range of bioactive secondary metabolites. We describe a versatile procedure for systematic and comprehensive mutagenesis of the S. coelicolor genome. The high-throughput process relies on in vitro transposon mutagenesis of an ordered cosmid library; mutagenized cosmids with fully characterized insertions are then transferred by...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 15078860
DOI: 10.1101/gr.1710304
PMCID: PMC479117

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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