Large-Scale Transposition Mutagenesis of Streptomyces coelicolor Identifies Hundreds of Genes Influencing Antibiotic Biosynthesis

Abstract excerpt

ABSTRACT Gram-positive Streptomyces bacteria produce thousands of bioactive secondary metabolites, including antibiotics. To systematically investigate genes affecting secondary metabolism, we developed a hyperactive transposase-based Tn 5 transposition system and employed it to mutagenize the model species Streptomyces coelicolor , leading to the identification of 51,443 transposition insertions. These...

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