Article
Construction of gene interruptions and gene deletions in the cyanobacterium Synechocystis sp. strain PCC 6803.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
Eaton-Rye Julian J
Abstract excerpt
A series of protocols are presented for the storage, growth, transformation, and characterization of wild type and mutant strains of Synechocystis sp. strain PCC 6803. These protocols include the isolation of genomic DNA and the strategies required for the construction of specific gene interruptions or deletions in this organism. This cyanobacterium has been used widely as a model for photosynthesis research, and...
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