Article
Combinatorial selection for replicable RNA by Qβ replicase while maintaining encoded gene function.
PloS one - 1 Jan 2017
Yumura Mio, Yamamoto Natsuko, Yokoyama Katsushi, Mori Hirotada, Yomo Tetsuya, Ichihashi Norikazu
Abstract excerpt
Construction of a complex artificial self-replication system is challenging in the field of in vitro synthetic biology. Recently, we developed a translation-coupled RNA replication system, wherein an artificial genomic RNA replicates with the Qβ RNA replicase gene encoded on itself. The challenge is to introduce additional genes into the RNA to develop a complex system that mimics natural living systems. However,...
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