Article
The construction of gene knockouts in the cyanobacterium Synechocystis sp. PCC 6803.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2004
Eaton-Rye Julian J
Abstract excerpt
A series of protocols are presented for the storage, growth, transformation, and characterization of wild-type (wt) and mutant strains of Synechocystis PCC 6803. These protocols include the isolation of genomic DNA and the strategies required for the construction of specific gene knockouts in this organism. This cyanobacterium has been used widely as a model for photosynthesis research and the sequence of its...
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