Article
Dynamics of Golgi matrix proteins after the blockage of ER to Golgi transport.
Journal of biochemistry - 1 Feb 2004
Yoshimura Shin-ichiro, Yamamoto Akitsugu, Misumi Yoshio, Sohda Miwa, Barr Francis A, Fujii Gourou, Shakoori Abbas, Ohno Hiroshi, Mihara Katsuyoshi, Nakamura Nobuhiro
Abstract excerpt
When the ER to Golgi transport is blocked by a GTP-restricted mutant of Sar1p (H79G) in NRK-52E cells, most Golgi resident proteins are transported back into the ER. In contrast, the cis-Golgi matrix proteins GM130 and GRASP65 are retained in punctate cytoplasmic structures, namely Golgi remnants...
Topics
- Autoantigens
- Biological Transport
- Cells, Cultured
- Cytoplasm
- Endoplasmic Reticulum
- Golgi Apparatus
- Membrane Proteins
- Microinjections
- Microscopy, Electron
- Monomeric GTP-Binding Proteins
- Mutation
