Article
Golgi membrane dynamics after induction of a dominant-negative mutant Sar1 GTPase in tobacco.
Journal of experimental botany - 1 Jan 2010
Osterrieder Anne, Hummel Eric, Carvalho Claudine M, Hawes Chris
Abstract excerpt
An inducible system has been established in Nicotiana tabacum plants allowing controlled expression of Sar1-GTP and thus the investigation of protein dynamics after inhibition of endoplasmic reticulum (ER) to Golgi transport. Complete Golgi disassembly and redistribution of Golgi markers into the ER was observed within 18-24h after induction. At the ultrastructural level Sar1-GTP expression led to a decrease in...
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