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Genetic Methods and Construction of Chromosomal Mutations in Methanogenic Archaea.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2022

Thomsen Johanna, Weidenbach Katrin, Metcalf William W, Schmitz Ruth A

Abstract excerpt

Genetic manipulation through markerless exchange enables the modification of several genomic regions without leaving a selection marker in the genome. Here, a method using hpt coding for hypoxanthine phosphoribosyltransferase as a counter selectable marker is described. For Methanosarcina species a chromosomal deletion of the hpt gene is firstly generated, which confers resistance to the purine analogue...

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PMID: 36125745
DOI: 10.1007/978-1-0716-2445-6_6

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