Article
Application of the split-ubiquitin membrane yeast two-hybrid system to investigate membrane protein interactions.
Methods (San Diego, Calif.) - 1 Apr 2004
Fetchko Michael, Stagljar Igor
Abstract excerpt
The characterization of protein-protein interactions provides the foundation for further studies concerning protein complex function and regulation. Since the advent of the yeast two-hybrid assay, many additional genetic systems based upon the principle of protein fragment complementation have been designed. One such system, the split-ubiquitin membrane yeast two-hybrid system (MbYTH), is able to analyze the...
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