Article
The mammalian-membrane two-hybrid assay (MaMTH) for probing membrane-protein interactions in human cells.
Nature methods - 1 May 2014
Petschnigg Julia, Groisman Bella, Kotlyar Max, Taipale Mikko, Zheng Yong, Kurat Christoph F, Sayad Azin, Sierra J Rafael, Mattiazzi Usaj Mojca, Snider Jamie, Nachman Alex, Krykbaeva Irina, Tsao Ming-Sound, Moffat Jason, Pawson Tony, Lindquist Susan, Jurisica Igor, Stagljar Igor
Abstract excerpt
Cell signaling, one of key processes in both normal cellular function and disease, is coordinated by numerous interactions between membrane proteins that change in response to stimuli. We present a split ubiquitin-based method for detection of integral membrane protein-protein interactions (PPIs) in human cells, termed mammalian-membrane two-hybrid assay (MaMTH). We show that this technology detects stimulus...
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