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Article

In vivo fragment complementation of a (beta/alpha)(8) barrel protein: generation of variability by recombination.

FEBS letters - 27 Feb 2004

Soberón Xavier, Fuentes-Gallego Patricia, Saab-Rincón Gloria

Abstract excerpt

The high representation of the TIM barrel as a scaffold for enzymatic proteins makes it an interesting model for protein engineering. Based on previous reports of folding mechanisms of TIM barrels that suggest an independent folding unit formed by six (beta/alpha) subunits, we interrupted the gene of phosphoribosylanthranilate isomerase (PRAI) from Escherichia coli at three different positions to yield fragments...

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PMID: 14988017
DOI: 10.1016/S0014-5793(04)00098-5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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