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Article

Optimized and automated protocols for high-throughput screening of amylosucrase libraries.

Journal of biomolecular screening - 1 Aug 2007

Emond Stéphane, Potocki-Véronèse Gabrielle, Mondon Philippe, Bouayadi Khalil, Kharrat Hakim, Monsan Pierre, Remaud-Simeon Magali

Abstract excerpt

This article describes the design and validation of a general procedure for the high-throughput isolation of amylosucrase variants displaying higher thermostability or increased resistance to organic solvents. This procedure consists of 2 successive steps: an in vivo selection that eliminates inactive variants followed by automated screening of active variants to isolate mutants displaying enhanced features. The...

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PMID: 17517906
DOI: 10.1177/1087057107301978

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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