Article
Oligosaccharide and sucrose complexes of amylosucrase. Structural implications for the polymerase activity.
The Journal of biological chemistry - 6 Dec 2002
Skov Lars K, Mirza Osman, Sprogøe Desiree, Dar Imran, Remaud-Simeon Magali, Albenne Cecile, Monsan Pierre, Gajhede Michael
Abstract excerpt
The glucosyltransferase amylosucrase is structurally quite similar to the hydrolase alpha-amylase. How this switch in functionality is achieved is an important and fundamental question. The inactive E328Q amylosucrase variant has been co-crystallized with maltoheptaose, and the structure was determined by x-ray crystallography to 2.2 A resolution, revealing a maltoheptaose binding site in the B'-domain somewhat...
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