Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Combinatorial engineering of dextransucrase specificity.

PloS one - 1 Jan 2013

Irague Romain, Tarquis Laurence, André Isabelle, Moulis Claire, Morel Sandrine, Monsan Pierre, Potocki-Véronèse Gabrielle, Remaud-Siméon Magali

Abstract excerpt

We used combinatorial engineering to investigate the relationships between structure and linkage specificity of the dextransucrase DSR-S from Leuconostoc mesenteroides NRRL B-512F, and to generate variants with altered specificity. Sequence and structural analysis of glycoside-hydrolase family 70 enzymes led to eight amino acids (D306, F353, N404, W440, D460, H463, T464 and S512) being targeted, randomized by...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Dextrans
Share to Glucans
Share to Glucosyltransferases

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Glycosyltransferases

Share to Leuconostoc

Share to Magnetic Resonance Spectroscopy

Share to Peptide Library

Share to Protein Engineering

Source

Open in PubMed Open full text

Identifiers

PMID: 24204991
DOI: 10.1371/journal.pone.0077837
PMCID: PMC3799614

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Combinatorial engineering of dextransucrase specificity.PMID 24204991Read ↗

Characterization of the inserted mutagenesis dextransucrases from Leuconostoc mesenteroides 0326 to produce hyperbranched dextran.Semantic proximity Read ↗

Mutations in the Different Residues between Dextransucrase Gtf-DSM and Reuteransucrase GtfO for the Investigation of Linkage Specificity Determinants.Semantic proximity Read ↗

Engineering Leuconostoc mesenteroides dextransucrase by inserting disulfide bridges for enhanced thermotolerance.Semantic proximity Read ↗

Applying pairwise combinations of amino acid mutations for sorting out highly efficient glucosylation tools for chemo-enzymatic synthesis of bacterial oligosaccharides.Semantic proximity Read ↗

Maximization of dextransucrase activity expressed in E. coli by mutation and its functional characterization.Semantic proximity Read ↗

Increased amylosucrase activity and specificity, and identification of regions important for activity, specificity and stability through molecular evolution.Semantic proximity Read ↗

The thermoduric effects of site-directed mutagenesis of proline and lysine on dextransucrase from Leuconostoc mesenteroides 0326.Semantic proximity Read ↗

Mutational analysis and characterization of dextran synthesizing enzyme from wild and mutant strain of Leuconostoc mesenteroides.Semantic proximity Read ↗

Engineering the glucansucrase GTFR enzyme reaction and glycosidic bond specificity: toward tailor-made polymer and oligosaccharide products.Semantic proximity Read ↗

The Discovery, Molecular Cloning, and Characterization of Dextransucrase LmDexA and Its Active Truncated Mutant from Leuconostoc mesenteroides NN710.Semantic proximity Read ↗

Computer-aided engineering of a branching sucrase for the glucodiversification of a tetrasaccharide precursor of S. flexneri antigenic oligosaccharides.Semantic proximity Read ↗

Combinatorial engineering to enhance amylosucrase performance: construction, selection, and screening of variant libraries for increased activity.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.