Combining a Base Deaminase Mutator with Phage-Assisted Evolution.

Abstract excerpt

Phage-assisted evolution has emerged as a powerful technique for improving a protein's function by using mutagenesis and selective pressure. However, mutations typically occur throughout the host's genome and are not limited to the gene-of-interest (GOI): these undesirable genomic mutations can yield host cells that circumvent the system's selective pressure. Our system targets mutations specifically toward the...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Bacteriophage T7
• Share to Bacteriophages
• Share to Mutagenesis