Article
Functional properties of subunit interactions in human cytidine deaminase.
Protein engineering - 1 Dec 2003
Vincenzetti Silvia, De Sanctis Giampiero, Costanzi Stefano, Cristalli Gloria, Mariani Pierluigi, Mei Giampiero, Neuhard Jan, Natalini Paolo, Polzonetti Valeria, Vita Alberto
Abstract excerpt
An intersubunit interactions study related to the active site has been performed on the wild-type cytidine deaminase (CDA) and on the mutant enzyme F137W/W113F. F137 is the homologous to the Bacillus subtilis CDA F125 involved in the subunit interactions. In the presence of SDS, wild-type human CDA dissociates into enzymatically inactive monomers without intermediate forms via a non-cooperative transition....
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