Article
An SSB encoded by and operating on linear killer plasmids from Kluyveromyces lactis.
Yeast (Chichester, England) - 30 Sept 2001
Schaffrath R, Meacock P A
Abstract excerpt
The Kluyveromyces lactis linear plasmids k1 and k2 belong to the family of protein-primed linear DNA genomes, which includes adenoviruses. Here we identify the 18 kDa gene product of k2ORF5 as a novel putative single-stranded DNA binding protein, SSB. As judged from Western analysis using an epitope-tagged fusion protein and ssDNA-agarose affinity chromatography, the Orf5 protein preferentially binds to ssDNA in...
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