Article
Rapid high-efficiency site-directed mutagenesis by the phosphorothioate approach.
BioTechniques - 1 Oct 1992
Sayers J R, Krekel C, Eckstein F
Abstract excerpt
Several improvements to the existing phosphorothioate-based site-directed mutagenesis methodology are reported, and here it is demonstrated that the new procedure is able to produce large deletions, insertions and point mutations rapidly and with very high efficiency. The time required for the polymerization step has been reduced by using T7 DNA polymerase to extend the mutant oligonucleotide primer-template. The...
Topics
- Base Sequence
- DNA
- DNA-Directed DNA Polymerase
- Exodeoxyribonucleases
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Organothiophosphorus Compounds