Article
c-Fos activated phospholipid synthesis is required for neurite elongation in differentiating PC12 cells.
Molecular biology of the cell - 1 Apr 2004
Gil Germán A, Bussolino Daniela F, Portal Maximiliano M, Alfonso Pecchio Adolfo, Renner Marianne L, Borioli Graciela A, Guido Mario E, Caputto Beatriz L
Abstract excerpt
We have previously shown that c-Fos activates phospholipid synthesis through a mechanism independent of its genomic AP-1 activity. Herein, using PC12 cells induced to differentiate by nerve growth factor, the genomic effect of c-Fos in initiating neurite outgrowth is shown as distinct from its nongenomic effect of activating phospholipid synthesis and sustaining neurite elongation. Blocking c-Fos expression...
Topics
- Animals
- Blotting, Western
- Cell Differentiation
- Cell Division
- Cell Nucleus
- Cytoplasm
- Dose-Response Relationship, Drug
- Electrophoresis, Polyacrylamide Gel
- Gene Deletion
- Microscopy, Fluorescence
- Mutation
- Neurons
- PC12 Cells