Article
PC12nnr5 cells expressing TrkA receptors undergo morphological but not cholinergic phenotypic differentiation in response to nerve growth factor.
Journal of neurochemistry - 1 Feb 2002
Baskey Jacqueline C, Kalisch Bettina E, Davis Wanda L, Meakin Susan O, Rylett R Jane
Abstract excerpt
We investigated mechanisms underlying nerve growth factor-mediated morphological differentiation and expression of cholinergic neuronal phenotype. In PC12, but not PC12nnr5 cells, nerve growth factor induces neurite-like outgrowths and enhances cholinergic phenotype; stable expression of TrkA receptors in nnr5 cells (called B5P cells) restores morphological differentiation but not expression of choline...
Topics
- Acetylcholine
- Animals
- Cell Differentiation
- Choline O-Acetyltransferase
- Electrophoretic Mobility Shift Assay
- Gene Expression Regulation, Enzymologic
- Genes, Reporter
- Nerve Growth Factor
- Neurons
- Nitric Oxide
- PC12 Cells
- Phenotype
- Proto-Oncogene Proteins c-fos