Article
Hyperactive initiation of chromosomal replication in vivo and in vitro by a mutant initiator protein, DnaAcos, of Escherichia coli.
The Journal of biological chemistry - 29 Apr 1994
Katayama T, Kornberg A
Abstract excerpt
DnaA protein initiates genomic replication in Escherichia coli. A cold-sensitive dnaAcos mutant caused excessive initiation at a restrictive temperature without an increase in the level of DnaA protein. The chromosomal origin (oriC) was essential for the lethality caused by the dnaAcos product. I...
Topics
- Bacterial Proteins
- Bacteriophage phi X 174
- Cell Division
- Chromosomes, Bacterial
- Cold Temperature
- DNA Replication
- DNA, Bacterial
- DNA, Single-Stranded
- DNA, Viral
- DNA-Binding Proteins
- Escherichia coli
- Genetic Complementation Test