Article
Characterization of an N6 adenine methyltransferase from Helicobacter pylori strain 26695 which methylates adjacent adenines on the same strand.
The FEBS journal - 1 Apr 2010
Kumar Ritesh, Mukhopadhyay Asish K, Rao Desirazu N
Abstract excerpt
Genomic sequences of Helicobacter pylori strains 26695, J99, HPAGI and G27 have revealed an abundance of restriction and modification genes. hp0050, which encodes an N(6) adenine DNA methyltransferase, was cloned, overexpressed and purified to near homogeneity. It recognizes the sequence 5'-GRRG-3' (where R is A or G) and, most intriguingly, methylates both adenines when R is A (5'-GAAG-3'). Kinetic analysis...
Topics
- Adenine
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA Methylation
- DNA Primers
- Dose-Response Relationship, Drug
- Helicobacter pylori
- Kinetics
- Molecular Sequence Data
- Mutation
- Peptides
- Sequence Homology, Amino Acid
- Site-Specific DNA-Methyltransferase (Adenine-Specific)
- Spectrometry, Fluorescence