The rate of hydrolytic deamination of 5-methylcytosine in double-stranded DNA.

doi:10.1093/nar/22.6.972

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Article

The rate of hydrolytic deamination of 5-methylcytosine in double-stranded DNA.

Nucleic acids research - 25 Mar 1994

Shen J C, Rideout W M, Jones P A

Abstract excerpt

The modified base, 5-methylcytosine, constitutes approximately 1% of human DNA, but sites containing 5-methylcytosine account for at least 30% of all germline and somatic point mutations. A genetic assay with a sensitivity of 1 in 10(7), based on reversion to neomycin resistance of a mutant pSV2-...

Topics

5-Methylcytosine
Base Sequence
Cytosine
DNA
DNA-Cytosine Methylases
Deamination
Hydrolysis
Kinetics
Methylation
Mutagenesis, Site-Directed
Mutation
Plasmids
Thermodynamics
Transformation, Genetic

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