Article
Novel TP53 gene mutations in tumors of Russian patients with breast cancer detected using a new solid phase chemical cleavage of mismatch method and identified by sequencing.
Human mutation - 1 Feb 2004
Lambrinakos A, Yakubovskaya M, Babon J J, Neschastnova A A, Vishnevskaya Ya V, Belitsky G A, D'Cunha G, Horaitis O, Cotton R G H
Abstract excerpt
Mutations in the tumor-suppressor p53 gene TP53 are frequent in most human cancers including breast cancer. A new solid phase chemical cleavage of mismatch method (CCM) allowed rapid and efficient screening and analysis of the TP53 gene in DNA samples extracted from tumors of 89 breast cancer patients. The novel CCM technique utilized silica beads and the potassium permanganate/tetraethylammonium chloride...
Topics
- Adult
- Aged
- Base Pair Mismatch
- Breast Neoplasms
- DNA Mutational Analysis
- DNA, Neoplasm
- Female
- Genes, p53
- Humans
- Middle Aged
- Mutation
- Nucleic Acid Heteroduplexes
- Russia