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Controlled, Scalable Embryonic Stem Cell Differentiation Culture

1 May 2004

Abstract excerpt

Embryonic stem (ES) cells are of significant interest as a renewable source of therapeutically useful cells. ES cell aggregation is important for both human and mouse embryoid body (EB) formation and the subsequent generation of ES cell derivatives. Aggregation between EBs (agglomeration), however, inhibits cell growth and differentiation in stirred or high-cell-density static cultures. We demonstrate that the...

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PMID: 15153605
DOI: 10.1634/stemcells.22-3-275

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