Article
A novel dysfunctional p53 mutation in the human neuroblastoma cell line TGW.
The Tohoku journal of experimental medicine - 1 Dec 2003
Sugiyama Hisahiko, Arita Michitsune, Min Zhenghua, Zhong Xioling, Iwasaki Iwao, Hirano Keihachiro, Shimatake Hiroyuki, Hemmi Hiromichi
Abstract excerpt
Mutations of p53 are rare in primary and advanced neuroblastomas. The p53 gene was studied in a TGW cell line established from a TNB1 xenograft, derived from metastasized neuroblastoma. The p53 protein level in TGW was elevated at baseline. Treatment with doxorubicin to induce genotoxic stress neither altered the p53 protein level nor induced p21 protein within 24 hours. DNA sequencing analysis revealed a novel...
Topics
- Apoptosis
- Cell Line, Tumor
- Codon
- DNA Mutational Analysis
- Disease Progression
- Genes, Reporter
- Genes, p53
- Humans
- Immunohistochemistry
- Mutation
- Neuroblastoma