Article
Use of transcription reporters with novel p53 binding sites to target tumour cells expressing endogenous or virally transduced p53 mutants with altered sequence-specificity.
Oncogene - 5 Feb 1998
Gagnebin J, Kovar H, Kajava A V, Estreicher A, Jug G, Monnier P, Iggo R
Abstract excerpt
p53 triple mutants (120N/121G/277H, 120H/121G/ 277H, 120S/121G/277H and 120H/121G/277Y) have altered sequence specificity in bandshift assays in vitro and transcription assays in vivo. These mutants activate transcription from the site TTT CATG AAA but not from wild type sites. The triple mutants...
Topics
- Base Sequence
- Binding Sites
- Consensus Sequence
- DNA, Neoplasm
- Genes, Reporter
- Genes, p53
- Genetic Therapy
- Humans
- Luciferases
- Mutation
- Neoplasms
- Sarcoma, Ewing
- Substrate Specificity
- Transcription, Genetic
- Transcriptional Activation