Article
Generation of a large number of connective tissue type mast cells by culture of murine fetal skin cells.
The Journal of investigative dermatology - 1 Dec 2003
Yamada Nobuo, Matsushima Hironori, Tagaya Yutaka, Shimada Shinji, Katz Stephen I
Abstract excerpt
We describe a novel culture system for generating large numbers of murine skin-associated mast cells and distinguish their characteristics from bone marrow-derived cultured mast cells. Culture of day 16 fetal skin single cell suspensions in the presence of interleukin-3 and stem cell factor allowed expansion and maturation of mast cells in the presence of stromal cells. The average yield of mast cells after 2 wk...
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