Article
Buffy coat preparation is a convenient source of progenitors for culturing mature human mast cells.
Journal of immunological methods - 20 Feb 2006
Wang X S, Yip K H, Sam S W, Lau H Y A
Abstract excerpt
Mast cells are unique immune cells that release a spectrum of chemical mediators contributing to the inflammatory symptoms of allergic disorders. Mature mast cells have recently been cultured from CD34(+) progenitors isolated from fresh umbilical cord blood and adult peripheral blood. In the current study, we investigated whether buffy coat preparations, which are readily available from blood banks, could be used...
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