Article
Genotyping of single nucleotide polymorphisms using base-quenched probe: a method does not invariably depend on the deoxyguanosine nucleotide.
Analytical biochemistry - 15 Mar 2009
Luo Guanghua, Zheng Lu, Zhang Xiaoying, Zhang Jun, Nilsson-Ehle Peter, Xu Ning
Abstract excerpt
Most available methods for detecting single nucleotide polymorphisms (SNPs) are based principally on the system that can produce an increased fluorescence signal during hybridization. In the current study, we demonstrate a method of base-quenched probe for polymerase chain reaction (PCR) genotyping that requires only a pair of primers and one fluorescent probe and does not invariably depend on the deoxyguanosine...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.